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IDs: 10494541 [UID] 27856948 [GenBank] 37153318 [RefSeq]
The assembly fErpCal1.3 is based on 58x PacBio data, 31x 10X Genomics Chromium data, and 35x Arima Hi-C data generated at the Wellcome Sanger Institute, as well as BioNano Saphyr DLE data generated at the Rockefeller University Vertebrate Genome ... Laboratory. The assembly process included the following sequence of steps: initial PacBio assembly generation with Falcon-unzip, retained haplotig separation with purge_dups, 10X based scaffolding with scaff10x, BioNano hybrid-scaffolding with Solve, Hi-C based scaffolding with SALSA2, Arrow polishing using Merfin, and two rounds of FreeBayes polishing. Finally, the assembly was analysed and manually improved using gEVAL. The mitochondrial assembly was produced at The Rockefeller University using mitoVGP. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. The GC profile of chromosome 11 is very unusual when compared with the other chromosomes. more
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