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IDs: 3286621 [UID] 10890548 [GenBank] 11512478 [RefSeq]
The assembly fSalTru1.1 is based on 68x PacBio data and 70x coverage 10X Genomics Chromium data generated at the Wellcome Sanger Institute, as well as BioNano data generated at the Rockefeller University Vertebrate Genome Laboratory and 28x coverage Hi-C ... data generated by Arima Genomics. The assembly process included the following sequence of steps: initial PacBio assembly generation with Falcon-unzip, retained haplotig identification with Purge Haplotigs, 10X based scaffolding with scaff10x, BioNano hybrid-scaffolding, Hi-C based scaffolding with SALSA2, Arrow polishing, and two rounds of FreeBayes polishing. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds confirmed by the Hi-C data have been named on the following basis: first they were assigned as metacentric versus acrocentric based on a Salmo trutta linkage map and synteny with the Salmo salar assembly GCA_000233375.4; numbers 1 to 11 are metacentric, numbers 12 to 40 are acrocentric, and within each of those two categories they are named in order of size. more
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