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IDs: 7920811 [UID] 21712188 [GenBank] 32592718 [RefSeq]
The assembly mSciCar1.2 is based on 74x PacBio data, 40x 10X Genomics Chromium data, and 42x Dovetail Hi-C data generated at the Wellcome Sanger Institute. The assembly process included the following sequence of steps: initial PacBio assembly generation with ... Falcon-unzip, retained haplotig identification with purge_dups, 10X based scaffolding with scaff10x, Hi-C based scaffolding with SALSA2, Arrow polishing, and two rounds of FreeBayes polishing. The mitochondrial assembly was produced at The Rockefeller University using mitoVGP. Finally, the assembly was analysed and manually improved using gEVAL. Since Hi-C data were sparse, curation was aided by synteny with the assembly for Sciurus vulgaris simultaneously being curated by the Wellcome Sanger Institute. Chromosome-scale scaffolds confirmed by the Hi-C data and synteny have been named in order of size. more
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