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IDs: 8865041 [UID] 23988778 [GenBank] 24303048 [RefSeq]
The assembly sScyCan1.1 is based on 63x PacBio data, 43x 10X Genomics Chromium data, and BioNano data generated at the Wellcome Sanger Institute, and 17x Hi-C data generated at the Baylor College of Medicine. The assembly process included the ... following sequence of steps: initial PacBio assembly generation with Falcon-unzip, retained haplotig identification with purge_dups, 10X based scaffolding with scaff10x, BioNano hybrid-scaffolding, Hi-C based scaffolding and improvement by the Aiden lab as part of a collaboration with DNA Zoo using 3D-DNA and Juicebox, Arrow polishing, and two rounds of FreeBayes polishing. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. more
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