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IDs: 7304321 [UID] 20180748 [GenBank] 42844348 [RefSeq]
The assembly fDanAes4.1 is based on 52x PacBio data, 71x 10X Genomics Chromium data, BioNano data and 32x Dovetail Hi-C data generated at the Wellcome Sanger Institute. The assembly process included the following sequence of steps: initial PacBio assembly ... generation with Falcon-unzip, retained haplotig separation with purge_dups, 10X based scaffolding with scaff10x, BioNano hybrid-scaffolding with Solve, Hi-C based scaffolding with SALSA2, Arrow polishing, and two rounds of FreeBayes polishing. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds are named by synteny to the GRCz11 assembly of Danio rerio. more
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