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IDs: 12133561 [UID] 31798448 [GenBank] 33407918 [RefSeq]
The assembly iyBomTerr1.2 is based on 57x PacBio data, 10X Genomics Chromium data, and Arima Hi-C data generated by the Darwin Tree of Life Project (https://www.darwintreeoflife.org/). The assembly process included the following sequence of steps: initial PacBio assembly generation ... with Hifiasm, retained haplotig separation with purge_dups, short-read polishing using FreeBayes-called variants from 10X Genomics Chromium reads aligned with LongRanger, and Hi-C based scaffolding with SALSA2. The mitochondrial genome was assembled using MitoHifi. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds are named by synteny based on Bombus terrestris (buff-tailed bumblebee) GCA_000214255.1. Many unplaced scaffolds are composed entirely of a 10mer repeat which we note occurs in other Bombus assemblies. more
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