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IDs: 10700101 [UID] 28357308 [GenBank] 32592368 [RefSeq]
The assembly iVesVel2.1 is based on 56x PacBio data and 94x 10X Genomics Chromium data generated at the Wellcome Sanger Institute, and Hi-C data generated at the Baylor College of Medicine. The assembly process included the following sequence of ... steps: palindromic read correction with pbclip, initial PacBio assembly generation with Falcon-unzip, retained haplotig separation with purge_dups, Hi-C based scaffolding with SALSA2, Arrow polishing, and short-read polishing using FreeBayes-called variants from 10X Genomics Chromium reads aligned with LongRanger. The mitochondrial assembly was produced using mitoVGP. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. more
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