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IDs: 11638071 [UID] 30643598 [GenBank] 30942488 [RefSeq]
The assembly mMelMel3.1 is based on 85x PacBio data, and Arima Hi-C data generated by the Darwin Tree of Life Project (https://www.darwintreeoflife.org/). The assembly process included the following sequence of steps: HiFi reads were assembled using Hifiasm and k-mers from ... the Illumina-sequenced parents in 'trio' mode; segregation of HiC reads using Canu's 'splitHaplotype' script and k-mers from the Illumina-sequenced parents; for each haplotype assembly, haplotype-specific and unclassified HiC reads were combined to construct scaffolds using SALSA2. The mitochondrial genome was assembled using MitoHifi. Finally, the assembly was analysed and manually improved using gEVAL, combining the paternal haplotype with the X chromosome from the maternal haplotype. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. more
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