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IDs: 12473881 [UID] 32657238 [GenBank] 35295828 [RefSeq]
The assembly dhQueRobu3.1 is based on 37x PacBio data, 10X Genomics Chromium data, and Arima Hi-C data generated by the Darwin Tree of Life Project (https://www.darwintreeoflife.org/). The assembly process included the following sequence of steps: initial PacBio assembly generation ... with Hifiasm, retained haplotig separation with purge_dups, short-read polishing using FreeBayes-called variants from 10X Genomics Chromium reads aligned with LongRanger, and Hi-C based scaffolding with SALSA2. The mitochondrial and chloroplast genomes were assembled with MBG from PacBio HiFi reads mapping to related genomes. A representative circular sequence was selected for each from the graph based on read coverage. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds are named by synteny based on PMID: 27013549. more
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