Herein, we report the new long-read assembly of Darmor-bzh genome (Brassica napus) generated by combining long-read sequencing data and optical and genetic maps. Using the PromethION device and 6 flowcells, we generated ∼16 million long reads representing 93× coverage and, more importantly, 6× with reads longer than 100 kb. This ultralong-read dataset allows us to generate one of the most contiguous and complete assemblies of a Brassica genome to date (contig N50 > 10 Mb). In addition, we exploited all the advantages of the nanopore technology to detect modified bases and sequence transcriptomic data using direct RNA to annotate the genome and focus on resistance genes.
| Accession | PRJEB75266 |
| Type | Umbrella project |
| Publications | Rousseau-Gueutin M et al., "Long-read assembly of the Brassica napus reference genome Darmor-bzh.", Gigascience, 2020 Dec 15;9(12) |
| Submission | Registration date: 26-Apr-2024
Genoscope |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| Nucleotide (total) | 106 |
| WGS master | 1 |
| Genomic DNA | 19 |
| SRA Experiments | 9 |
| Protein Sequences | 108170 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 3 |
| Assembly | 1 |
This project encompasses the following 3 sub-projects:
| Project Type | Number of Projects |
Genome sequencingHighest level of assembly : Chromosomes |
1
|
BioProject
accession | Assembly
level | Name | Title |
|---|
| PRJEB40412 | Chromosomes | Long-reads assembly and annotation of the Brassica napus reference genome, Darmor-bzh | Long-reads assembly and annotation of the Brassica napus reference genome, Darmor-bzh (Genoscope CEA) |
|
| Other | 2 |
BioProject
accession | Name | Title |
|---|
| PRJEB39416 | CCM | Brassica napus reference genome, Darmor-bzh sequencing data (Genoscope) | | PRJEB39508 | CHY | Direct RNA sequencing of the transcriptome of Brassica napus Darmor-bzh (Genoscope) |
|