Masan National Tuberculosis Hospital1 (MNTH) is one of the implementation portions of Korea's National Tuberculosis Protocols, which focuses on the treatment of multidrug-resistant (MDR) and extensively drug-resistant (XDR) TB patients.
More...Masan National Tuberculosis Hospital1 (MNTH) is one of the implementation portions of Korea's National Tuberculosis Protocols, which focuses on the treatment of multidrug-resistant (MDR) and extensively drug-resistant (XDR) TB patients. MNTH started prescribing LZD to its patients with rifampicin-resistance or MDR, according to guidelines set by both the hospital and the government2, 3. The phenotypic drug susceptibility test of first- and second-line anti-tuberculosis drugs were determined by the Sensititre plate, and REMA settled the MIC of linezolid. A total sequential isolate previously collected and deposited to the Masan National TB Hospital Biobank was used in this study. All of these were from four MDR/XDR patients who failed linezolid-included treatment. All WGS of the isolates were performed with the Illumina platform (MiSeq system, Illumina, Inc.). DNAs were isolated and WGS for mutation identification and genetic determinants of resistance sequencing of the whole genome at the loci that were commented or reported drug-resistant.
1. Biobank for multidrug-resistant tuberculosis research: the importance of sequential samples. Pathog Dis. 2021 Mar 20;79(3):ftab011.
2. Prevention KCDC. Korean Guidelines for Tuberculosis, Third Edition, 2017. 2017.
3. Hospital MNT. The MNTH Guide to the Management of Tuberculosis. 2020. Less...