BioProject

Chinese hamster ovary (CHO) cells serve as the predominant recombinant protein production host across the biotechnology industry. More...

Chinese hamster ovary (CHO) cells serve as the predominant recombinant protein production host across the biotechnology industry. Characteristics such as glycosylation, fast growth, and ease of genetic manipulation help explain CHO prevalence. Since its establishment, CHO has diverged into different adherent and suspension cell lines, such as CHO-K1, CHO-S, and CHO DG44. Despite the wide applicability of CHO to research and development, we have relied upon limited draft genome sequences from 2011 and 2013 for transcriptomics, proteomics, genetic engineering, and other research. To aid future CHO efforts, an improved genome utilizing increased read lengths is desirable to fill gaps in the current genome. Specifically, PacBio technology was used to generate long reads from Chinese hamster liver tissue. Subsequently, the PacBio data was combined with previous Illumina short read data (also derived from Chinese hamster liver) to further improve the Chinese hamster genome assembly. Evaluation of four assembly strategies incorporating the short and long read data was used to assess and improve genome completeness. This meta-assembly of these four strategies represents the most complete Chinese hamster genome to-date and can be used as a reference across the biotech industry. Less...