BioProject

The genome of the red flour beetle, Tribolium castaneum, was sequenced more than ten years ago using Sanger sequencing of BAC and fosmid libraries of a highly inbred strain, GA2, and assembled using Atlas suite. More...

The genome of the red flour beetle, Tribolium castaneum, was sequenced more than ten years ago using Sanger sequencing of BAC and fosmid libraries of a highly inbred strain, GA2, and assembled using Atlas suite. The genome was significant as it was the first sequenced genome of a major agricultural pest and the first coleopteran species sequenced. However, despite its wide utility in functional genomics and integration into genetic models, the current assembly (Tcas5.2) lacks approximately 35% of the total base pairs of the 205 Mbp genome, and the sequence of the Y chromosome remains elusive, likely due to highly repetitive sequences. We therefore attempted to improve the assembly with additional high coverage (140x) long-read sequence from GA2 and long-range information from Hi-C data (218M read pairs of 2x151 bp, 210,294x coverage). Less...