BioProject

Here, we present the whole genome sequencing of five Thermus thermophilus HB27 strains used to study the physiological role of Ttago and TthPrimpol in DNA interference. More...

Here, we present the whole genome sequencing of five Thermus thermophilus HB27 strains used to study the physiological role of Ttago and TthPrimpol in DNA interference. The starting wild type strain is T. thermophilus HB27 (ATCC BAA-163/DSM7039), which was used to generate the strain HB27 Δprimpol, a markerless deletion mutant of TT_C0656 loci, generated by cre – lox system. Additionally, a second HB27 Δprimpol (called HB27 Δprimpol Clone 2) was isolated from an independent colony of the same parental strain and using the same cre – lox system.On the other hand, we generated from the strain T. thermophilus HB27EC, the mutant HB27EC Δago, a markerless deletion mutant of TT_P0026 loci, obtained by homologous recombination and further loss of the antibiotic resistant cassette. The last mutant strain is HB27EC ΔagoΔprimpol, a double mutant employing T. thermophilus HB27EC Δago as parental strain and using cre – lox system to delete TT_C0656 loci.HB27, HB27 Δprimpol, HB27EC Δago and HB27EC ΔagoΔprimpol were transformed prior sequencing with plasmid pMoTK_3A (5) to quantify plasmid copy number. HB27 Δprimpol Clone 2 was not transformed with the plasmid. Less...