BioProject

This study used a combined RNA-Seq and Tn-Seq approach to characterize the response of Enterohemmorhagic E. More...

This study used a combined RNA-Seq and Tn-Seq approach to characterize the response of Enterohemmorhagic E. coli (EHEC) to metabolites from an in vitro culture of 33 human gut microbiota isolates (MET-1). The dataset deposited here contains sequencing reads of a Tn-seq compatible EHEC mariner transposon mutant library which was created using the transposon donor plasmid pSAM-Ec. The Tn-seq library was cultured in media containing microbiota metabolites or control rich and minimal media before sequencing to identify genes essential for growth in response to the gut microbiota metabolites. Less...