Replicative RNA enables directed evolution and Dar... (ID 985713) - BioProject

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Accession: PRJNA985713 ID: 985713

Replicative RNA enables directed evolution and Darwinian adaptation in mammalian cells

This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series

Accession	PRJNA985713; GEO: GSE235343
Type	Umbrella project
Submission	Registration date: 20-Jun-2023 Peking University
Relevance	Superseries

Project Data:

Parameter	Value
Data volume, Supplementary Mbytes	175

Parameter	Value
Data volume, Gbases	213
Data volume, Mbytes	90425

Replicative RNA enables directed evolution and Darwinian adaptation in mammalian cells encompasses the following 14 sub-projects:

Project Type

Number of Projects

Other

BioProject accession	Organism	Title
PRJNA985719	Mesocricetus auratus	Analysis of the accumulation of mutations in self-replicating RNAs using barcoded EGFP library (Peking University)
PRJNA985697	Mesocricetus auratus	Analyzing the process of mutation accumulation in MEK1 evolution experiment using amplicon sequencing (Peking University)
PRJNA985699	Mesocricetus auratus	Analyzing the process of mutation accumulation in KRAS evolution experiment using amplicon sequencing (Peking University)
PRJNA1133794	Mesocricetus auratus	Escaping from a dominant‐negative KRAS using REPLACE (Peking University)
PRJNA1133798	Mesocricetus auratus	Quantification of induced RNA mutation by nucleoside analogs in the repRNA-v4 EGFP region (Peking University)

Transcriptome or Gene expression

BioProject accession	Organism	Title
PRJNA985720	Mesocricetus auratus	Single cell RNA-seq of BHK-21 cells carrying alphaviral RNA replication system (Peking University)
PRJNA985721	Mesocricetus auratus	Measurement of the degradation rate of alphaviral RNA in BHK-21 cells (Peking University)
PRJNA898802	Mesocricetus auratus	RNA sequencing of BHK-21 cells carrying alphaviral RNA replication system (Peking University)
PRJNA1133793	Mesocricetus auratus	Evolution of drug resistance enabled by replicative RNAs carrying MEK1 (Peking University)
PRJNA1133799	Mesocricetus auratus	Quantification of basal RNA mutation rate in the repRNA-v4 EGFP region (Peking University)