Cortical neurogenesis follows a simple lineage: apical Radial Glia Cells (RGC) generate basal progenitors, and these produce neurons. How this occurs in species with expanded germinal zones and a folded cortex, like human, remains unclear. We used single-cell RNA sequencing from individual cortical germinal zones in ferret, and barcoded lineage tracking, to determine the molecular diversity of progenitor cells and their lineages. We identified multiple RGC classes that initiate parallel lineages, converging onto a common class of newborn neuron. Parallel RGC classes and transcriptomic trajectories were repeated across germinal zones and conserved in ferret and human, but not mouse. Neurons followed parallel differentiation trajectories in gyrus and sulcus, with different expression of human cortical malformation genes.
More...Cortical neurogenesis follows a simple lineage: apical Radial Glia Cells (RGC) generate basal progenitors, and these produce neurons. How this occurs in species with expanded germinal zones and a folded cortex, like human, remains unclear. We used single-cell RNA sequencing from individual cortical germinal zones in ferret, and barcoded lineage tracking, to determine the molecular diversity of progenitor cells and their lineages. We identified multiple RGC classes that initiate parallel lineages, converging onto a common class of newborn neuron. Parallel RGC classes and transcriptomic trajectories were repeated across germinal zones and conserved in ferret and human, but not mouse. Neurons followed parallel differentiation trajectories in gyrus and sulcus, with different expression of human cortical malformation genes. Progenitor cell lineage multiplicity is conserved in the folded mammalian cerebral cortex.
This SuperSeries is composed of the SubSeries listed below.
Overall design: Refer to individual Series
Single-cell RNA sequencing (Borrell lab):
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE219254
Barcoded lineage tracking (Mayer lab):
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE233587
Less...| Accession | PRJNA980720; GEO: GSE234305 |
| Type | Umbrella project |
| Publications | - Del-Valle-Anton L et al., "Multiple parallel cell lineages in the developing mammalian cerebral cortex.", Sci Adv, 2024 Mar 29;10(13):eadn9998
- Singh A et al., "Gene regulatory landscape of cerebral cortex folding.", Sci Adv, 2024 Jun 7;10(23):eadn1640
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| Submission | Registration date: 6-Jun-2023
Borrell Lab, Instituto de Neurociencias, Consejo Superior de Investigaciones CientÃficas & Universidad Miguel Hernández |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 26 |
| Publications |
| PubMed | 2 |
| PMC | 2 |
| Other datasets |
| BioSample | 26 |
| GEO DataSets | 3 |
Multiple parallel cell lineages in the developing mammalian cerebral cortex encompasses the following 2 sub-projects:
| Project Type | Number of Projects |
| Transcriptome or Gene expression | 2 |
BioProject
accession | Organism | Title |
|---|
| PRJNA907812 | Mustela putorius | scRNA-seq of ferret cortical germinal zones from splenial gyrus and lateral sulcus (Borrell lab) (Borrell Lab, Instituto de...) | | PRJNA976704 | Mustela putorius furo | Barcode lineage tracking of ferret cortical progeny using TrackerSeq (Mayer lab) (Borrell Lab, Instituto de...) |
|