In this study, we performed an analysis of the virome of 51 grapevine plants from the Don Ampelographic Collection named after Ya. I. Potapenko (Russia) using high-throughput se-quencing of the total RNA. A total of 20 previously described grapevine viruses and 4 viroids were identified. The most widespread were grapevine rupestris stem pitting-associated virus (98%), hop stunt viroid (98%), grapevine Pinot gris virus (96%), grapevine yellow speckle viroid 1 (94%), and grapevine fleck virus (GFkV, 80%). Among the economically significant viruses, the most widespread were grapevine leafroll-associated virus 3 (37%), grapevine virus A (24%), and grapevine leafroll-associated virus 1 (16%). For the first time in Russia, we detected grape-vine-associated tymo-like virus (78%). After a bioinformatics analysis, 123 complete or nearly complete viral genomes and 64 complete viroid genomes were assembled. An analysis of phy-logenetic relationships with existing global isolates was performed. We discovered and charac-terized the genomes of five novel grapevine viruses: bipartite dsRNA grapevine alphapartitivirus (genus Alphapartitivirus, family Partitiviridae), bipartite (+) ssRNA grapevine secovirus (genus Fa-bavirus, family Secoviridae) and three (+) ssRNA grapevine umbra-like viruses 2, -3, -4 (which oc-cupy an intermediate position between representatives of the genus Umbravirus and umbravi-rus-like associated RNAs).
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