With the aim of understanding the mutations that caused the downregulation or the synthesis of non-functional OprD porin, we sequenced two P. aeruginosa strains that presented a resistance profile compatible with alterations in OprD porin expression, including in the analysis the two supposed parental strains.
P. aeruginosa TNP004, for decades known as an OprD mutant, was previously found to carry a single nucleotide substitution in OprD. We therefore wanted to ascertain that this was the only cause of the non-functional protein synthesis.
P. aeruginosa LG03 was found by western-blot analysis to have a downregulation in OprD expression, and we were interested to understand the underlying mechanism.
PAO1-Jap and ARC545 were two P. aeruginosa PAO1 strains, received from distinct laboratories, that were found to both carry mutations in a region upstream the opdP gene. Interestingly, OpdP is a porin involved in antibiotic uptake as OprD. Less...