Expression profiling Hawaiian Drosophila species, ... (ID 317989) - BioProject

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Accession: PRJNA317989 ID: 317989

Expression profiling Hawaiian Drosophila species, tissues, and sexes

This is a prepublication dataset that should change as updates are made to the underling genomes and annotations. More...

This is a prepublication dataset that should change as updates are made to the underling genomes and annotations. Additional samples may be deposited and some samples may fail quality control. Please contact Haiwang Yang for details. The RNA-seq data set contains the transcriptional profiling of eight sexed tissue types (head, thorax, carcass, abdomen w/o gonad, gonad, other reproductive tract, genitalia, and digestive system) and/or whole adult flies from five strains of Drosophila grimshawi, two strains of D. silvestris, and one strain each of D. hemipeza, D. heteroneura, and D. hawaiiensis. Overall design: Samples were collected in duplicate to quadruplicate from sexually mature sexed adults (4-5 weeks post eclosion), which had been allowed to freely mate. Single-end stranded 76bp PolyA+ RNA-seq was performed on all samples. Libraries were multiplexed with indexed adaptors, and some were also prepared as mixed libraries with RNA from genetically distant species: Drosophila melanogaster (GSE81142) and Anopheles stephensi (GEO entry in preparation). All whole adult samples were prepared from single flies. Tissues were pooled from 3-5 individuals depending on the size of both the tissue and the species. For the G1 strain (reference assembly strain) of D. grimshawi, we profiled seven adult tissues for each sex in addition to whole flies. Samples were whole fly (one fly per sample), head (5 flies), thorax (5 flies), abdomen w/o gonad (5 flies), gonad (testes or ovaries) (5 flies), other reproductive tract (accessory gland and male reproductive duct or female spermatheca and oviduct; 10 flies per sample), genitalia (10 flies), and digestive system (proventriculus, crop, salivary gland, gut, and renal tubule; 5 flies). We prepared sexed single fly whole body samples in quadruplicate for ZA27Z2, ZA27Z3, and Mo010 strains of D. grimshawi. We also prepared sex-specific head, carcass, gonad, other reproductive tract with genitalia with duplicate for Wm1041 strain of D. grimshawi and other species - D. silvestris (Y11R6 and U26B9 strains), D. hemipeza (W40B14 strain), D. heteroneura (W48B6 strain), and D. hawaiiensis (Y17P5 strain). We used reduced number of flies for the non-grimshawi species (i.e., 3 heads, gonads or carcasses per sample, and 6 reproductive tracts plus genitalia per sample). Less...

Accession	PRJNA317989; GEO: GSE80124
Data Type	Transcriptome or Gene expression
Scope	Multispecies
Publications	Yang H et al., "Re-annotation of eight Drosophila genomes.", Life Sci Alliance, 2018 Dec;1(6):e201800156
Submission	Registration date: 11-Apr-2016 Developmental Genomics, LCDB, NIDDK, NIH
Relevance	Unknown

Project Data:

Resource Name	Number of Links
Sequence data
SRA Experiments	341
Publications
PubMed	1
PMC	1
Other datasets
BioSample	345
GEO DataSets	1

GEO Data Details

Parameter	Value
Data volume, Supplementary Mbytes	1365

SRA Data Details

Parameter	Value
Data volume, Gbases	345
Data volume, Tbytes	0.23

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Expression profiling Hawaiian Drosophila species, tissues, and sexes

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