To isolate genome-wide expression genes from peanut, a high yield and quality peanut cultivar (Arachis hypogaea cv Minhua6) was used for gene discovery. Eight tissues from root, stem, leaf, flower, peg, pod perecarp, testa and embryo of different developing stages and young seedlings were used for RNA extraction. Equally mixed RNA was used to prepare cDNA library.
Additionally, samples subjected to biotic and abiotic stress, which included low temperature and drought, low Ca2+, hormone treatments (ethylene, abscisic acid, salicylic acid and paclobutracol), and inoculation with Aspergillus flavus and Rolstonia solanacearum and their corresponding controls were taken for RNA extraction. Then equally mixed stressed RNAs and mixed control RNAs were used to prepare two cDNA libraries, a stressed cDNA and a control cDNA library. cDNA libraries were then subjected to 454 XLR70 Titanium Pyrosequencing, respectively. 10 Additionally, samples subjected to biotic and abiotic stress, which included low temperature and drought, low Ca2 , hormone treatments (ethylene, abscisic acid, salicylic acid and paclobutracol), and inoculation with Aspergillus flavus and Rolstonia solanacearum and their corresponding controls were taken for RNA extraction. Then equally mixed stressed RNAs and mixed control RNAs were used to prepare two cDNA libraries, a stressed cDNA and a control cDNA library. cDNA libraries were then subjected to 454 XLR70 Titanium Less...