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Aurelia aurita associated microbial sample

Identifiers
BioSample: SAMEA6087651; SRA: ERS3888057
Organism
Aurelia aurita (moon jelly)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Cnidaria; Scyphozoa; Semaeostomeae; Ulmaridae; Aurelia
Attributes
collection date2016-06-28
broad-scale environmental contextmarine biome
local-scale environmental contextfjord
environmental mediumsea water
geographic locationBaltic Sea
host body habitatwhole animal
host body productwhole animal
host tissue sampledwhole animal
host subject idAaurita_1
investigation typemetagenome
organism count1
project nameComparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms
sample collection device or methodDOI:10.1186/s40168-019-0743-1
sample material processingDOI:10.1186/s40168-019-0743-1
sample nameG05140-L1
16s_primerF-CCTACGGGAGGCAGCAG;R-GGACTACHVGGGTWTCTAAT
ENA-CHECKLISTERC000014
ENA-FIRST-PUBLIC2019-10-31T04:04:09Z
ENA-LAST-UPDATE2019-10-16T20:46:36Z
External IdSAMEA6087651
INSDC center nameMPIEVB
INSDC first public2019-10-31T04:04:09Z
INSDC last update2019-10-16T20:46:36Z
INSDC statuspublic
Submitter IdG05140-L1
amount or size of sample collected1 individual
amplification protocolV3V4-two step
common namemoon jelly
environmentaquatic
geographic location (latitude)54.462654
geographic location (longitude)9.842743
geographic location (region and locality)Baltic Sea
host-associated environmental packagehost-associated
human associated environmental packagehuman-associated
library construction methodPCR
multiplex identifiersi5-GCGTAAGA;i7-TGCCTCTT
nucleic acid extractionDOI:10.1186/s40168-019-0743-1
pcr conditions1. step:initial denaturation:98degC_30s;25times;denaturation:98degC_10s;annealing:55degC_30s;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity; 2. step:initial denaturation:98degC_30s;10times;denaturation:98degC_9s;annealing:55degC_30s;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity
pcr primersstep 1 F-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCGATCCCTACGGGAGGCAGCAG;step 1 R-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTCTCGGACTACHVGGGTWTCTAAT;step 2 F-AATGATACGGCGACCACCGAGATCTACACGCGTAAGATCGTCGGCAGCGTC;step 2 R-CAAGCAGAAGACGGCATACGAGATTGCCTCTTGTCTCGTGGGCTCGG
scientific_nameAurelia aurita
sequencing methodIllumina
target gene16S rRNA
target subfragmentV3V4
Description

Aurelia aurita medusa associated microbiota after IGEPAL

BioProject
PRJEB30924 Amplicon and metagenomic sequencing reveals key features in metaorganisms
Retrieve all samples from this project

Submission
EBI; 2019-11-01
Accession:
SAMEA6087651
ID:
13177858

BioProject SRA

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