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CCFI 777453.Guillardia.theta.2712.cDNA (H)

Identifiers
BioSample: SAMN00167596; EST: LIBEST_024596
Organism
Guillardia theta
cellular organisms; Eukaryota; Cryptophyceae; Pyrenomonadales; Geminigeraceae; Guillardia
Attributes
strainCCMP2712 (single-cell isolate of CCMP327)
lab hostDH10B
vectorpCMV-SPORT6
re_1SalI
re_2NotI
Description

The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

Submission
DOE Joint Genome Institute, Lindquist,E.A.; 2009-05-12
Accession:
SAMN00167596
ID:
167596

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