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GNK

Identifiers
BioSample: SAMN00184239; GSS: LIBGSS_011167
Organism
Zea mays
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
Attributes
cultivarmix background A188/W23/B73/K55
tissueLeaf
development stageadult
lab hostDH10B
tissueLeaf
vectorMightyMu_ACT (engineered from pBluescript)
v_typeplasmid
re_1BamHI
re_2BglII
Description

MightyMu_ACT is a 5.3 kb, modified maize Mu1 transposon designed for activation tagging and to allowed plasmid rescue from total genomic DNA. Total leaf DNA was double digested using BamHI and BglII, and ligated to form circular plasmids. DH10B cells were transformed and then screened on LB plates with ampicillin.

Submission
Stanford University, Nan GL; 2007-10-24
Accession:
SAMN00184239
ID:
184239

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