MIGS Eukaryotic sample from Aphanomyces cochlioides

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Result Filters

Identifiers: BioSample: SAMN18761261; Sample name: 103-1_Illumina_RNA_10dpi; SRA: SRS8713938

Organism: Aphanomyces cochlioides
cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Aphanomyces

Package: MIGS: eukaryote, plant-associated; version 6.0

Attributes

isolate	103-1
collection date	2014-12-01
broad-scale environmental context	temperate biome
local-scale environmental context	agricultural field
environmental medium	agricultural soil
geographic location	USA: Minnesota
host	Beta vulgaris
isolation and growth condition	Soil baiting
latitude and longitude	46.26 N 96.58 W

Description

Tissue from A. cochlioides infected sugar beet seedlings were collected for RNA extraction with the RNeasy Mini Kit for Plants (Qiagen Inc., Valencia, CA, USA). 2-week old sugar beet seedlings (American Crystal 093RR) were inoculated with 5,000 zoospores per plant, and diseased roots were removed from pots at 1, 3, 5, and 10 days post inoculation (dpi) for RNA extraction, and Illumina sequencing.

Keywords: GSC:MIxS;MIGS:6.0