Canfam_toti_minion_v1_9_20�C21120�C9_macherey_nage... - BioSample

Canfam_toti_minion_v1_9_20�C21120�C9_macherey_nagel_MN4_bc08

Identifiers: BioSample: SAMEA113986600; SRA: ERS15970484

Organism: Canis lupus familiaris (dog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis; Canis lupus

Attributes

broad-scale environmental context	Csongrad county
collection date	2021-12-01
environmental medium	no
geographic location	Hungary
host age	13
host body product	stool
host common name	Hungarian herding terrier: Pumi
host diet	omnivore
host family relationship	Harsanyi-Tekergo Bator
host	Canis lupus familiaris
host sex	male
host subject id	not applicable
host subspecific genetic lineage	cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxonomy ID	9615 (9615)
organism count	not collected
project name	Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
sample name	Canfam_Toti_MinION_MachN_4
sample storage duration	96
ENA-CHECKLIST	ERC000013
ENA-FIRST-PUBLIC	2023-12-15T20:42:06Z
ENA-LAST-UPDATE	2023-12-15T20:42:06Z
External Id	SAMEA113986600
INSDC center name	UNIVERSITY OF SZEGED
INSDC first public	2023-12-15T20:42:06Z
INSDC last update	2023-12-15T20:42:06Z
INSDC status	public
Submitter Id	Canfam_Toti_MinION_MachN_4
adapters	ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG; ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT
chimera check software	EMU
common name	dog
geographic location (latitude)	46.248755
geographic location (longitude)	20.145766
library construction method	16S Barcoding Kit (SQK-RAB20�C4)
library screening strategy	ligation based
local environmental context	Szeged
multiplex identifiers	16SF(ATCGCCTACCGTGAC / barcode / AGAGTTTGATCMTGGCTCAG); 16SR(ATCGCCTACCGTGAC / barcode / CGGTTACCTTGTTACGACTT)
nucleic acid amplification	LongAmp Taq 2X Master Mix
nucleic acid extraction	Macherey Nagel NucleoSpin DNA Stool kit
pcr conditions	initial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min
pcr primers	27F(AGAGTTTGATCCTGGCTCAG); 1492R(GGTTACCTTGTTACGACTT)
scientific_name	Canis lupus familiaris
sequence quality check	software
sequencing method	Oxford Nanopore, SQK-RAB20�C4
target gene	16S rRNA
target subfragment	V1-V9

Description: Dog stool sample of Toti

BioProject: PRJEB59610 Metagenomic profiling of canine gut microbial community
Retrieve all samples from this project

Submission: EBI; 2023-12-17

Accession:: SAMEA113986600
ID:: 38864886

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BioSample

National Center for Biotechnology Information

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Canfam_toti_minion_v1_9_20�C21120�C9_macherey_nagel_MN4_bc08

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