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Canfam_toti_pacbio_v1_v9_20�C220�C722_macherey_nagel_MN1

Identifiers
BioSample: SAMEA113986625; SRA: ERS15970509
Organism
Canis lupus familiaris (dog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis; Canis lupus
Attributes
broad-scale environmental contextCsongrad county
collection date2021-12-01
environmental mediumno
geographic locationHungary
host age13
host body productstool
host common nameHungarian herding terrier: Pumi
host dietomnivore
host family relationshipHarsanyi-Tekergo Bator
hostCanis lupus familiaris
host sexmale
host subject idnot applicable
host subspecific genetic lineagecross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host taxonomy ID9615 (9615)
organism countnot collected
project nameComparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
sample nameCanfam_Toti_PacBio_MachN_1
sample storage duration96
sub speciesfamiliaris
ENA-CHECKLISTERC000013
ENA-FIRST-PUBLIC2023-12-15T20:42:06Z
ENA-LAST-UPDATE2023-12-15T20:42:06Z
External IdSAMEA113986625
INSDC center nameUNIVERSITY OF SZEGED
INSDC first public2023-12-15T20:42:06Z
INSDC last update2023-12-15T20:42:06Z
INSDC statuspublic
Submitter IdCanfam_Toti_PacBio_MachN_1
adaptersCACGACACGACGATGT; ATGTGCGTGTGTGTCT
chimera check softwareEMU
common namedog
geographic location (latitude)46.248755
geographic location (longitude)20.145766
library construction methodsoftware
library screening strategyligation based
local environmental contextSzeged
multiplex identifiers16S_Fw_1020(CACGACACGACGATGT); 16S_Rev_1056(ATGTGCGTGTGTGTCT)
nucleic acid amplificationLongAmp Taq 2X Master Mix
nucleic acid extractionMacherey Nagel NucleoSpin DNA Stool kit
pcr conditionsinitial denaturation:95degC_1min; denaturation=95degC_20�Csec; annealing=55deg_30sec; extension=65deg_2min
pcr primers27F(AGRGTTTGATYMTGGCTCAG); 1492R(GGYTACCTTGTTACGACTT)
scientific_nameCanis lupus familiaris
sequence quality checksoftware
sequencing methodPacBio RS II SMRT DNA Sequencing System, P6-C4
target gene16S rRNA
target subfragmentV1-V9
Description

Dog stool sample of Toti

BioProject
PRJEB59610 Metagenomic profiling of canine gut microbial community
Retrieve all samples from this project

Submission
EBI; 2023-12-17
Accession:
SAMEA113986625
ID:
38865048

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