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toti_minion_WGS_20230802_invitrogen_I1

Identifiers
BioSample: SAMEA115615481; Sample name: toti_minion_WGS_20230802_invitrogen_I1; SRA: ERS19893061
Organism
Canis lupus familiaris (dog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis; Canis lupus
Attributes
broad-scale environmental contextCsongrad county
hostCanis lupus familiaris
collection date2021-12-01
host family relationshipHarsanyi-Tekergo Bator
host subject idnot applicable
host subspecific genetic lineagecross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host common nameHungarian herding terrier: Pumi
host sexmale
temperature20
geographic locationHungary
sample storage temperature-80
host taxonomy ID9615 (9615)
environmental mediumno
host dietomnivore
sample storage durationn.a
host body productstool
organism countnot collected
project nameUltra-deep, hybrid metagenomic sequencing of canine gut microbiome
host age13 years
sub speciesfamiliaris
chimera check softwareminitax
sequence quality checkLongQC
target genewhole genome
geographic location (longitude)20.14824
nucleic acid extractionPureLink Microbiome DNA Purification Kit, Invitrogen
nucleic acid amplificationLongAmp Taq 2X Master Mix
library reads sequencednot provided
adaptersTop strand: 5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3' / Bottom strand: 5'-ACGTAACTGAACGAAGTACAGG-3'
organismCanis lupus familiaris
library construction methodNative Barcoding Kit 24 V14 (SQK-NBD114.24)
library sizenot provided
geographic location (latitude)46.253
scientific_nameCanis lupus familiaris
common namedog
target subfragmentnot applicable
pcr primersnot applicable
pcr conditionsno pcr
local environmental contextSzeged
sequencing methodOxford Nanopore, SQK-NBD114.24
library screening strategyligation based
multiplex identifiersCACAAAGACACCGACAACTTTCTT
Description

Dog stool sample of Toti

BioProject
PRJEB75753 Ultra-deep, hybrid metagenomic sequencing of canine gut microbiome
Retrieve all samples from this project

Submission
EBI; 2024-07-27
Accession:
SAMEA115615481
ID:
42853088

BioProject SRA

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