Control 12 h

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Result Filters

Identifiers: BioSample: SAMD00003848; SRA: DRS005716

Organism: Sorghum bicolor (sorghum)
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum

Attributes

sample name	DRS005716
sample comment	Sorghum (Sorghum bicolor L. Moench) SIL-05, which is resistant to target leaf spot, was used for mRNA seq. Four seeds were sterilized, placed onto Kumiai-Ryujyou-Baido soil (Kureha Chemical, Tokyo, Japan) in each plant box (5Ã5Ã15 cm high) and incubated in a chamber for 7 days under 16 h light and 8 h dark at 28Â°C. On day 7 (about the 2- or 3-leaf stage) the sorghum plants were sprayed with 1 ml of 0.01% Tween-20 solution per plant box. The plants were sampled at 12 h after inoculation. Four biological replicates were collected, immediately frozen in liquid nitrogen, and mixed, to minimize the effect of transcriptome unevenness among plants. Total RNA from 4 plants was extracted with RNeasy (Qiagen, Hilden, Germany). The total RNA was subjected to further analysis. mRNA-seq library construction and sequencing were performed at Hokkaido System Science Co., Ltd. (Sapporo, Japan) in accordance with the manufacturer’s instructions by using an Illumina (San Diego, CA) TruSeq RNA Sample Prep Kit and 100-bp single-end protocol on a HiSeq2000 system (Illumina).

sample name

DRS005716

sample comment

Sorghum (Sorghum bicolor L. Moench) SIL-05, which is resistant to target leaf spot, was used for mRNA seq. Four seeds were sterilized, placed onto Kumiai-Ryujyou-Baido soil (Kureha Chemical, Tokyo, Japan) in each plant box (5Ã5Ã15 cm high) and incubated in a chamber for 7 days under 16 h light and 8 h dark at 28Â°C. On day 7 (about the 2- or 3-leaf stage) the sorghum plants were sprayed with 1 ml of 0.01% Tween-20 solution per plant box. The plants were sampled at 12 h after inoculation. Four biological replicates were collected, immediately frozen in liquid nitrogen, and mixed, to minimize the effect of transcriptome unevenness among plants. Total RNA from 4 plants was extracted with RNeasy (Qiagen, Hilden, Germany). The total RNA was subjected to further analysis. mRNA-seq library construction and sequencing were performed at Hokkaido System Science Co., Ltd. (Sapporo, Japan) in accordance with the manufacturer’s instructions by using an Illumina (San Diego, CA) TruSeq RNA Sample Prep Kit and 100-bp single-end protocol on a HiSeq2000 system (Illumina).