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Protocols: Sample tissue was taken from egg-laying female quail housed at the University of Zurich. We isolated RNA by homogenizing the tissue in TRIzol followed by DNase treatment and cleanup using Qiagen RNeasy columns according to manufacturer instructions. Libraries were constructed using the llumina TruSeq RNA Stranded mRNA kit according to standard protocols. RNA enrichment was done using the PolyA TrueSeq RNA Kit.
BioProject SRA
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