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Protocols: Cut of apical tissues, including early developping leaves and apical meristems, directly put in liquid nitrogen. Stages I : 1 week after germination (production of the first whorl of leaves); Stages II : 2 weeks after germination (second whorl of leaves); Stages III : 3 weeks after germination (third whorl of leaves); Stages IV : 6 weeks after germination (sexually mature individual) Plant growth in soil with 2 g of slow-release fertilizer per litre of soil Pools of tissues from 10 individuals. Total RNA extraction using RNeasy miny kit (Qiagen) TruSeq Stranded mRNA Sample Prep Kit (Illumina)
BioProject SRA
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