BioSample

The cDNA library was constructed, normalized and subtracted by the W.M. Keck Center, University of Illinois Urbana-Champaign as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. Messenger RNA extracted from Fusarium solani and soybean roots infected with Fusarium solani was converted to ds-cDNA with tagged oligo-dT used to identify the source of the ESTs where possible: ACGCA18(T) = Fusarium solani; TCCGA18(T) = soybean roots infected with Fusarium solani. The ds-cDNAs were size selected (more than 450 bp), adaptored with EcoRI adaptors and digested with NotI. The ds-cDNA were then directionally cloned into EcoRI-NotI digested pBluescriptII SK(+) phagemid vector. The normalized library was subtracted with cDNA synthesized from non-inoculated healthy soybean root mRNA. Clones were sequenced from the 3' end using standard primer M13 reverse n48.