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6_Psychrobacter_isolate_B

Identifiers
BioSample: SAMN28230942; Sample name: CCS169; SRA: SRS13027228
Organism
Psychrobacter sp. CCS169
cellular organisms; Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales; Moraxellaceae; Psychrobacter; unclassified Psychrobacter
Package
MIGS: cultured bacteria/archaea; version 6.0
Attributes
strainCCS169
collection date2020-08-20
broad-scale environmental contextanthropogenic environmental material[ENVO:0010001]
local-scale environmental contextcheese product[ENVO:00002189]
environmental mediumcheese rind biofilm
geographic locationUSA
isolation and growth conditionCCS169 was isolated from the rind of a washed-rind cheese by plating on PCAMS (5 g/L tryptone, 2.5 g/L yeast extract, 1g/L dextrose, 1 g/L whole milk powder, 10 g/L sodium chloride and 15 g/L agar) containing 100 g/ml cycloheximide to select against the fungal community members and incubation at 15 C for 4 days and an additional 3 days at room temperature on the bench top. A single colony was streak-purified on PCAMS three times. Overnight cultures of the isolate were grown for 24 hours at room temperature in LB Miller broth and flash-frozen in liquid nitrogen in PBS+20% glycerol. Glycerol stocks were stored at -80 C.
latitude and longitude32.8801 N 117.2340 W
number of repliconsn.a.
reference for biomaterialn.a.
Description

Keywords: GSC:MIxS;MIGS:6.0

BioProject
PRJNA837789
Retrieve all samples from this project

Submission
University of California San Diego, Christina Saak; 2022-05-13
Accession:
SAMN28230942
ID:
28230942

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