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H1_Bin_METABAT__97

Identifiers
BioSample: SAMEA14073058; SRA: ERS11676291
Organism
uncultured Aquificaceae bacterium
cellular organisms; Bacteria; Aquificota; Aquificia; Aquificales; Aquificaceae; environmental samples
Attributes
collection date2018-10-15
broad-scale environmental contextmarine biome [ENVO:00000447]
local-scale environmental contextmicrobial feature [ENVO:00002150]
environmental mediumMacroalgae
geographic locationChina
investigation typemetagenome-assembled genome
isolation sourceMariane macroalgea Gelidium sp.
project nameMetagenomes of marine macroalgae, surrounding seawater and surrounding sediments
sample nameH1_Bin_METABAT__97
ENA-CHECKLISTERC000011
ENA-FIRST-PUBLIC2023-01-18
ENA-LAST-UPDATE2023-01-18
External IdSAMEA14073058
INSDC center aliasMarine College, Shandong University
INSDC center namemarine college, shandong university
INSDC first public2023-01-18T00:18:47Z
INSDC last update2023-01-18T00:18:47Z
INSDC statuspublic
Submitter IdH1_Bin_METABAT__97
assembly qualityMany fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly softwareMEGAHIT v1.2.9
binning parametersanvi'o 6.2 default parameters, DASTOOL with --search-engine diamond
binning softwareCONCOCT, MAXBIN2,METABAT2, refined with dastool and manually with anvi'o 6.2
completeness score53.55
completeness softwarecheckM v1.1.3
contamination score2.05
geographic location (latitude)37.562
geographic location (longitude)122.121
metagenomic sourcemarine metagenome
sample derived fromERS10980522
sequencing methodIllumina Nova-PE150
taxonomic identity markermulti-marker approach (GTDB-tk v1.03, GTDB v95)
Description

Macroalgea were then put in sterile seawater and remaining bacteria were removed by ultrasonication (2x 60s, 50-60 kHz). Suspensions with removed bacteria of each sample were subsequently pooled with the previously obtained washing suspensions. Per sample, nearly 60 L of washing suspension was then filtered through 0.2 µm pore size polycarbonate membrane filters (Millipore). DNA was extracted from these filters, frozen at -80°C.

BioProject
PRJEB50838 undefined
Retrieve all samples from this project

Submission
EBI; 2023-01-19
Accession:
SAMEA14073058
ID:
32789431

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