H4_Bin_MAXBIN__028_sub_2

Organism: uncultured Sphingorhabdus sp.
cellular organisms; Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingorhabdus; environmental samples

Attributes

collection date	2019-08-01
broad-scale environmental context	marine biome [ENVO:00000447]
local-scale environmental context	microbial feature [ENVO:00002150]
environmental medium	Macroalgae
geographic location	China
investigation type	metagenome-assembled genome
isolation source	Mariane macroalgea Gelidium sp.
project name	Metagenomes of marine macroalgae, surrounding seawater and surrounding sediments
sample name	H4_Bin_MAXBIN__028_sub_2
ENA-CHECKLIST	ERC000011
ENA-FIRST-PUBLIC	2023-01-18
ENA-LAST-UPDATE	2023-01-18
External Id	SAMEA14073196
INSDC center alias	Marine College, Shandong University
INSDC center name	marine college, shandong university
INSDC first public	2023-01-18T00:18:51Z
INSDC last update	2023-01-18T00:18:51Z
INSDC status	public
Submitter Id	H4_Bin_MAXBIN__028_sub_2
assembly quality	Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software	MEGAHIT v1.2.9
binning parameters	anvi'o 6.2 default parameters, DASTOOL with --search-engine diamond
binning software	CONCOCT, MAXBIN2,METABAT2, refined with dastool and manually with anvi'o 6.2
completeness score	79.39
completeness software	checkM v1.1.3
contamination score	2.03
geographic location (latitude)	37.562
geographic location (longitude)	122.121
metagenomic source	marine metagenome
sample derived from	ERS10980525
sequencing method	Illumina Nova-PE150
taxonomic identity marker	multi-marker approach (GTDB-tk v1.03, GTDB v95)

Description: Macroalgea were then put in sterile seawater and remaining bacteria were removed by ultrasonication (2x 60s, 50-60 kHz). Suspensions with removed bacteria of each sample were subsequently pooled with the previously obtained washing suspensions. Per sample, nearly 60 L of washing suspension was then filtered through 0.2 µm pore size polycarbonate membrane filters (Millipore). DNA was extracted from these filters, frozen at -80°C.

BioSample