R4_Bin_MAXBIN__090_sub_1

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Organism: uncultured Bacteriovoracaceae bacterium
cellular organisms; Bacteria; Bdellovibrionota; Bacteriovoracia; Bacteriovoracales; Bacteriovoracaceae; environmental samples

Attributes

collection date	2019-08-01
broad-scale environmental context	marine biome [ENVO:00000447]
local-scale environmental context	microbial feature [ENVO:00002150]
environmental medium	Macroalgae
geographic location	China
investigation type	metagenome-assembled genome
isolation source	Mariane macroalgea Grateloupia sp.
project name	Metagenomes of marine macroalgae, surrounding seawater and surrounding sediments
sample name	R4_Bin_MAXBIN__090_sub_1
ENA-CHECKLIST	ERC000011
ENA-FIRST-PUBLIC	2023-01-18
ENA-LAST-UPDATE	2023-01-18
External Id	SAMEA14074105
INSDC center alias	Marine College, Shandong University
INSDC center name	marine college, shandong university
INSDC first public	2023-01-18T00:18:45Z
INSDC last update	2023-01-18T00:18:45Z
INSDC status	public
Submitter Id	R4_Bin_MAXBIN__090_sub_1
assembly quality	Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software	MEGAHIT v1.2.9
binning parameters	anvi'o 6.2 default parameters, DASTOOL with --search-engine diamond
binning software	CONCOCT, MAXBIN2,METABAT2, refined with dastool and manually with anvi'o 6.2
completeness score	92.56
completeness software	checkM v1.1.3
contamination score	0
geographic location (latitude)	37.562
geographic location (longitude)	122.121
metagenomic source	marine metagenome
sample derived from	ERS10980529
sequencing method	Illumina Nova-PE150
taxonomic identity marker	multi-marker approach (GTDB-tk v1.03, GTDB v95)

Description: Macroalgea were then put in sterile seawater and remaining bacteria were removed by ultrasonication (2x 60s, 50-60 kHz). Suspensions with removed bacteria of each sample were subsequently pooled with the previously obtained washing suspensions. Per sample, nearly 60 L of washing suspension was then filtered through 0.2 µm pore size polycarbonate membrane filters (Millipore). DNA was extracted from these filters, frozen at -80°C.

R4_Bin_MAXBIN__090_sub_1
R4_Bin_MAXBIN__090_sub_1

biosample
S1_Bin_METABAT__76_1
S1_Bin_METABAT__76_1

biosample
MAG: uncultured Saprospiraceae bacterium isolate R3_Bin_METABAT__71_sub_1, whole...
MAG: uncultured Saprospiraceae bacterium isolate R3_Bin_METABAT__71_sub_1, whole genome shotgun sequencing project
gi|2433125752|emb|CALIXU000000000.1 XU010000000

Nucleotide
MAG: uncultured Acidimicrobiales bacterium isolate H3_Bin_METABAT__239_1, whole ...
MAG: uncultured Acidimicrobiales bacterium isolate H3_Bin_METABAT__239_1, whole genome shotgun sequencing project
gi|2437496439|emb|CALGZB000000000.1 ZB010000000

Nucleotide
H1_Bin_METABAT__124_1_1
H1_Bin_METABAT__124_1_1

biosample

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