BioSample

Protocols: Tissue samples of multiple types were collected from adult individuals of zebrafish, medaka, and rainbow trout. Liver tissue samples were taken in replicates of four, or three in the case of rainbow trout. All fish were raised in fresh water under standard rearing conditions in aquaculture facilities (rainbow trout: Aquagen broodstock, tanks at NIVA solbergstranda), or animal laboratory facilities (zebrafish, medaka: VetBio, adamstuen). Total RNA was extracted from the tissue samples using the RNeasy Plus Universal Kit (QIAGEN). Quality was determined on a 2100 Bioanalyzer using the RNA 6000 Nano Kit (Agilent). Concentration was determined using a Nanodrop 8000 spectrophotometer (Thermo Scientific). cDNA libraries were prepared using the TruSeq Stranded mRNA HT Sample Prep Kit (Illumina). Library mean length was determined by running on a 2100 Bioanalyzer using the DNA 1000 Kit (Agilent) and library concentration was determined with the Qbit BR Kit (Thermo Scientific).