| depth | 351 m |
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| isolation source | groundwater from cold water geyser, filtered on 0.1um pore size filters |
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| collection date | 2018-02-21 |
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| geographic location | Germany: Volcanic Eifel, Andernach |
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| latitude and longitude | 50.448588 N 7.375355 E |
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| isolate name alias | GA_180221_E-1-2_metaspades_Caldisericum_37_73 |
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| broad-scale environmental context | groundwater|terrestrial subsurface|cold water geyser |
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| local-scale environmental context | cold water geyser|geyser eruption |
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| environmental medium | groundwater |
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| dissolved organic carbon | 1.39 (0.31) mg/L |
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| potassium | 3.15 (0.86) mM |
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| sulfate | 4.98 (0.39) mM |
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| calcium | 5.26 (0.21) mM |
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| chloride | 70.58 (7.26) mM |
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| sodium | 73.05 (2.28) mM |
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| magnesium | 9.05 (0.27) |
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| nitrate | BDL |
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| phosphate | BDL |
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| sulfide | BDL |
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| chemical administration | None |
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| conductivity | 12800-13600 S |
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| dissolved carbon dioxide | 5700 mg/L |
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| organism count | 3.5e10^6cells/mL total cells; eFM using DAPI |
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| oxygenation status of sample | anaerobic |
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| pH | 6.8 |
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| reference for biomaterial | https://www.biorxiv.org/content/10.1101/2020.03.07.980714v3.full.pdf |
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| sample collection device or method | 0.1 um pore size teflon filters |
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| sample material processing | DNA Powersoil max kit followed by EtOH precipitation with Glycogen carrier |
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| sample size | 30 L of filtered volume; 1/2 of filter was used for extraction |
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| sample storage duration | < 1 week |
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| sample storage location | - on dry ice (field) or in 80 C fridge (lab) |
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| sample storage temperature | -80 C |
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| sample volume or weight for DNA extraction | ~ 15 L (1/2 of a filter) |
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| size fraction selected | 0.1 m |
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| temperature | 18 C |
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| total depth of water column | 351 m |
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| total iron | 486.11 (39.06) M |
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| sample type | metagenomic assembly |
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| metagenome source | aquifer metagenome |
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| derived from | This BioSample is a metagenomic assembly obtained from the aquifer metagenome BioSample: SAMN14680029 |
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| isolate | GA_180221_E-1-2-MAG-37_73 |
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| metagenomic | 1 |
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| environmental-sample | 1 |
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