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Zebrafish Embryonic Heart cDNA Library

Identifiers
BioSample: SAMN00156144; EST: LIBEST_001568
Organism
Danio rerio (zebrafish)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes; Cyprinoidei; Danionidae; Danioninae; Danio
Attributes
development stageembryonic day 3 post-fertilization
lab hostE.coli XL1-Blue mrF'
tissueheart
vectorLambda ZAP Express
v_typePhage
re_1EcoRI
re_2XhoI
Description

mRNA was purified from embryonic zebrafish hearts (3 day post-fertilization). cDNA was synthesized using a XhoI-Oligo dT adaptor-primer. EcoRI adaptors were ligated, followed by digestion with XhoI, for directional cloning into pre-digested lambda ZAP Express vector.

Submission
Harvard Medical School, Liew CC; 1999-01-04
Accession:
SAMN00156144
ID:
156144

Nucleotide

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