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NCI_CGAP_Sub8 is a subtracted library derived from NCI_CGAP_Sub5. The NCI_CGAP_Sub8 library had 2.5 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub5 was used as a tracer in a subtractive hybridization with a driver comprising: a pool of clones from NCI_CGAP_Sub5 (IMAGE clone Ids 2732833-2737415, 3068040-3069191; 25% of the driver population), a pool of clones from NCI_CGAP_Sub4 (IMAGE clone Ids 2723592-2729326; 25% of the driver population), NCI_CGAP_Sub6 (pool AIF-AJU, IMAGE Ids 2728969-2733190; 25% of the driver population), and NCI_CGAP_Sub7 (IMAGE Ids 3069192-3072238, 3081864-3084550; 25% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.
Nucleotide
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