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Hembase; Erythroid Progenitor Cells (LCB:ax library)

Identifiers
BioSample: SAMN00163488; EST: LIBEST_008975
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
sexunknown
tissueblood
cell typeErythroid Cells
cell linePrimary Culture of Peripheral Blood Mononuclear Cells
development stageProgenitor; EPO responsive CD71++++
lab hostSOLR
tissueblood
vectorLambda ZAP II
v_typeplasmid
re_1EcoRI
re_2EcoRI
Description

65,000 proliferating erythroid cells from the buffy coat of a blood donation were obtained by flow cytometric separation after a 5-day culture period in the presence of erythropoietin. Total RNA was purified from the sorted cell population using TRIzol reagent. RNA (0.3 ug) was converted into double stranded cDNA using Clontech's CapFinder cDNA Library Construction Kit (Clontech) according to the manufacturer's protocol and cloned into EcoRI digested Lambda Zap II vector (Stratagene). The phage library was amplified once prior to in vivo excision in SOLR cells. Individual colonies were grown, and the cDNA inserts were sequenced in high throughput (NIH intramural sequencing center http://www.nisc.nih.gov/).

Submission
National Institute of Diabetes and Digestive and Kidney Diseases, Jeffery L. Miller; 2001-06-11
Accession:
SAMN00163488
ID:
163488

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