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Soares_NhHMPu_S1

Identifiers
BioSample: SAMN00155192; EST: LIBEST_000589
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuePooled human melanocyte, fetal heart, and pregnant uterus
lab hostDH10B
tissuemixed (see below)
vectorpT7T3D-PacI
v_typephagemid (ampicillin resistant)
re_1Not I
re_2Eco RI
Description

Equal amounts of plasmid DNA from three normalized libraries (melanocyte 2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 260232-265223, 340488-345479, and 484488-489479.

Submission
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Inge Arlart; 1996-11-27
Accession:
SAMN00155192
ID:
155192

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