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The UI-R-DM1 library is a normalized Rat Prostate library (nRPro) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996) The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch. The Rat Prostate tissue was provided by Tom Freeman of the Sanger Center.
Nucleotide
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