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UI-R-DM1

Identifiers
BioSample: SAMN00170064; EST: LIBEST_010151
Organism
Rattus norvegicus (Norway rat)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Attributes
strainSprague-Dawley
development stageadult
lab hostDH10B (Life Technologies)
vectorpT7T3D-PacI
v_typePhagemid
re_1Not I
re_2Eco RI
Description

The UI-R-DM1 library is a normalized Rat Prostate library (nRPro) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996) The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch. The Rat Prostate tissue was provided by Tom Freeman of the Sanger Center.

Submission
Children's Memorial Research Center, Soares, MB; 2002-01-17
Accession:
SAMN00170064
ID:
170064

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