UI-E-EO0

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Result Filters

Organism: Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo

Attributes

tissue	fetal eye
development stage	fetal
lab host	DH10B (Life Technologies) (T1 phage resistant)
tissue	eye
vector	pT7T3-Pac (Pharmacia) with a modified polylinker
v_type	Plasmid
re_1	EcoR I
re_2	Not I

Description: UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).