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High endothelial cells (HEC) were isolated from human tonsils using magnetic beads derivatized with MECA-79 antibody specific to the peripheral lymph node addressin. cDNA was prepared from these cells using Cap-Finder methodology, then cDNA prepared from tonsillar lymphocytes and from human umbilical vein endothelial cells were subtracted from the HEC cDNA, followed by normalization through selective PCR amplification.
Nucleotide
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