NCI_CGAP_FF0

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Result Filters

Organism: Rattus norvegicus (Norway rat)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus

Attributes

tissue	Mixed tissues
development stage	Adult
lab host	DH10B (Life Technologies) (T1 phage resistant)
vector	pT7T3-Pac (Pharmacia) with a modified polylinker
v_type	Plasmid
re_1	EcoR I
re_2	Not I

Description: UI-R-FF0 is a subtracted cDNA library containing the following tissue(s): Normal cartilage and SR-JWS Tumor Line . The subtraction was made according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for these libraries are: CTAATGGACG, CATTCTTGTA.