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955.fasta

Identifiers
BioSample: SAMEA112157006; SRA: ERS14266852
Organism
Bacillus subtilis
cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus subtilis group
Attributes
broad-scale environmental contextmarine biome [ENVO:00000447]
collection date2018-10-15
depth0
environmental mediummarine macroalgae
geographic locationChina
investigation typeisolate bacterial draft genome
project nameThis study represents the draft genome sequences of bacterial isolates retrieved from the phycosphere of marine macroalgae, surrounding seawater, and surrounding sediments at Weihai, China.
sample name955.fasta
ENA-CHECKLISTERC000024
ENA-FIRST-PUBLIC2023-01-18
ENA-LAST-UPDATE2023-01-18
External IdSAMEA112157006
INSDC center aliasMarine College, Shandong University
INSDC center namemarine college, shandong university
INSDC first public2023-01-18T00:19:23Z
INSDC last update2023-01-18T00:19:23Z
INSDC statuspublic
Submitter Id955.fasta
geographic location (latitude)37.562
geographic location (longitude)122.121
local environmental contextmicrobial feature [ENVO:00002150]
sequencing methodIllumina HiSeq-PE150
water environmental packageplant-associated
Description

Marine macroalgae, surrounding seawater, and surrounding sediment were collected at Weihai, China (122.12 N, 37.56 E). Phycosphere bacteria: Macroalgae were cut into pieces and rinsed thrice with sterile seawater. Afterwards, 10 g of these pieces were washed with 10 ml sterile seawater (rotary shaker, 170 rpm., 30 min, 25 °C). One-milliliter aliquots were diluted stepwise to 1:100,000 (sterile seawater), and 100 μl were subsequently plated and incubated (21 d, 28 °C). Seawater samples: One-milliliter seawater was diluted stepwise with 9 ml sterile seawater to 1:1,000. Aliquots of 100 μl were then plated and incubated as described above. Sediment samples: Sediment samples of 1 g were thoroughly mixed with 9 ml sterile seawater (rotary shaker, 170 rpm., 30 min, 25 °C) and then diluted in 1:10 steps with sterile seawater to 1:10,000. Afterwards, 100 μl aliquots were plated. Two media were used for plating, modified 2216E and modified VY/2 medium. Plating was completed within 2 h after sampling. After incubation, colony-forming units were counted with numbers ranging from 10 to 500. Colonies were selected depending on color, size, and shape. Picked colonies were purified by serial cultivation on plates with identical media. Purified strains were stored at -80 °C in a sterile 1% (w/v) saline medium with 15% (v/v) glycerol.

BioProject
PRJEB57783 undefined
Retrieve all samples from this project

Submission
EBI; 2023-01-19
Accession:
SAMEA112157006
ID:
32791331

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