Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Two different donor eyes (75-80 years old) yielded approximately 600 mg of dissected RPE/choroid tissue. This in turn yielded 340 ug of total RNA and 7 ug of mRNA. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies (Rockville, MD; now part of Invitrogen Corp), essentially following the protocols of the SuperScript Plasmid System (Invitrogen Corp. <http://www.invitrogen.com/>). The library code designation was cs. For this library, cDNA inserts were cloned into the NotI/MluI sites of the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).
Nucleotide
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on