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Single colony of Candidatus Chlorohelix allophototropha from enrichment culture (subculture 19.9)

Identifiers: BioSample: SAMN32083172; Sample name: Candidatus Chlorohelix allophototropha, single colony recovered from enrichment culture (subculture 19.9); SRA: SRS18088576

Organism: Candidatus Chlorohelix allophototropha
cellular organisms; Bacteria; Terrabacteria group; Chloroflexota; Chloroflexia; Candidatus Chloroheliales; Candidatus Chloroheliaceae; Candidatus Chlorohelix

Package: MIGS: cultured bacteria/archaea, water; version 6.0

Attributes

strain	L227-S17
collection date	2017-09
depth	3.88 m
broad-scale environmental context	small freshwater lake biome [ENVO_00000892]
local-scale environmental context	freshwater environment [ENVO_01000306]
environmental medium	anoxic water [ENVO_01000173]\|fresh water [ENVO_00002011]
geographic location	Canada: near Kenora, Ontario, Lake 227, IISD-Experimental Lakes Area
isolation and growth condition	https://doi.org/10.1101/2020.07.07.190934
latitude and longitude	49.69 N 93.69 W
number of replicons	5
reference for biomaterial	https://doi.org/10.1101/2020.07.07.190934
isolation source	Single colony of Candidatus Chlorohelix allophototropha from a non-axenic enrichment (subculture 19.9); enrichment culture was started from water from the upper anoxic zone (3.88 m depth) of an iron-rich Boreal Shield lake
type-material	reference material of Candidatus Chlorohelix allophototropha

Description

"Candidatus Chlorohelix allophototropha" is a mesophilic and phototrophic member of the Chloroflexota (formerly Chloroflexi) phylum cultivated from an iron-rich Boreal Shield lake (Canada). The species is currently (as of 2022) unique among the Chloroflexota phylum in its use of a Type I photosynthetic reaction center (RCI) to perform light energy conversion. Here, we picked a single, isolated colony of "Ca. Chx. allophototropha" from a non-axenic enrichment culture (subculture 19.9) and extracted its DNA. Based on 16S rRNA gene amplicon sequencing, only sequences of "Ca. Chx. allophototropha" were detectable in the sample (to a detection limit of ~0.03% relative abundance). Given that the sample was dominated by "Ca. Chx. allophototropha", we used the DNA from this sample to obtain the genome of "Ca. Chx. allophototropha".

Keywords: GSC:MIxS;MIGS:6.0