U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from Nucleotide

Soares_parathyroid_tumor_NbHPA

Identifiers
BioSample: SAMN00155028; EST: LIBEST_000417
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissueparathyroid tumor
development stageadult
lab hostDH10B (ampicillin resistant)
tissueparathyroid gland
vectorpT7T3D (Pharmacia) with a modified polylinker
v_typephagemid
re_1Not I
re_2Eco RI
Description

1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid adenomas was kindly provided by Dr. Stephen Marx, National Institute of Diabetes and Digestive and Kidney Diseases, NIH.

Submission
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Inge Arlart; 1995-12-07
Accession:
SAMN00155028
ID:
155028

Supplemental Content

Support Center